Products/Services Used | Details | Operation |
---|---|---|
PCR Cloning and Subcloning> | Subsequently, the IgG fraction from serum was purified by GenScript, Nanjing, China (Supplementary Fig. 6). The following antibodies were used for western blotting: NatD (Abcam; ab106408, 1:1000), GAPDH (MBL International; M171-3, 1:5000), Vimentin (BD Biosciences; 550513, 1:1000), E-cadherin (BD Biosciencs; 610181, 1:1000), N-cadherin (Abcam; ab76057, 1:1000), Slug (Abcam; ab27568, 1:500), Zeb1 (ABclonal; A5600, 1:1000), Zeb2 (Abcam; ab138222, 1:500), Twist1 (ABclonal; A7314, 1:1000), Snail (Santa Cruz Biotechnology; sc-271977, 1:500), Histone H4 (PTM Biolabs; PTM-1004, 1:2000), Histone H3(Genscript; A01502, 1:1000), H4K5ac (Millipore; CS204381, 1:1000), H4K8ac (Millipore; CS204357, 1:1000), H4K12ac (Millipore; 06-1352, 1:1000), H4R3me2a (Active Motif; 39705, 1:1000), H4R3me2s (Abcam; ab5823, 1:1000), H4S1ph (Abcam; ab14723, 1:1000), CK2α (Abcam; ab70774, 1:2000), Lamin A/C (Genscript; A01455, 1:2000), and Hsp70 (Genscript; A01236, 1:1000). | Get A Quote |
N-α-acetyltransferase D (NatD) mediates N-α-terminal acetylation (Nt-acetylation) of histone H4 known to be involved in cell growth. Here we report that NatD promotes the migratory and invasive capabilities of lung cancer cells in vitro and in vivo. Depletion of NatD suppresses the epithelial-to-mesenchymal transition (EMT) of lung cancer cells by directly repressing the expression of transcription factor Slug, a key regulator of EMT. We found that Nt-acetylation of histone H4 antagonizes histone H4 serine 1 phosphorylation (H4S1ph), and that downregulation of Nt-acetylation of histone H4 facilitates CK2α binding to histone H4 in lung cancer cells, resulting in increased H4S1ph and epigenetic reprogram... More